Many PLA signals were generated when ADAM10 and BACE1 were probed against both synaptophysin (Fig. 4a–d) and PSD-95 (Fig. 4e–h) in AD or control brain hippocampus, whereas only limited number of signals were detected in the negative control samples where the primary antibodies had been omitted (Fig. 4i–j). This evidence concerns the gene DLG4 and Alzheimer disease.