Positive controls demonstrated the expected staining pattern for OCT4 (Figure S5A) and NANOG (Figure S5B) in seminoma, SOX2 (Figure S5C) in normal skin, KLF4 (Figure S5D) in breast carcinoma, and c-MYC (Figure S5E) in normal colon. This evidence concerns the gene POU5F1 and breast carcinoma.