For the first time, we have investigated the effect of cis-bis(methylthio)silvatin on two colorectal adenocarcinoma cell lines with a different genetic profile; in fact, HCT116 expresses mutant β-catenin and K-RAS, while Caco-2 exhibits APC, β-catenin, and p53 gene mutations [20]. The gene discussed is APC; the disease is colorectal adenocarcinoma.