To determine whether increased HMGB1 level in peripheral nervous system is responsible for the painful neuropathy at four weeks after diabetes in animals, GLC was administered for five days a week for four weeks at a dose of 50 mg/kg per day I.P. ZDF animals that were treated with GLC showed significant alleviation of mechanical hyperalgesia (71.2 ± 9.1 gm; p < 0.01; Figure 1b). This evidence concerns the gene HMGB1 and diabetes mellitus.