As the results shown in Figure 5A, compared with HBV‐related HCC with BCLC staging between 0 and A, the mutation frequencies of COL12A1, TERT, c5orf42, CUBN, MDN1, OBSCN, EYS, FAT2, and HSPG2 were increased in patients with BCLC staging between B and C. In addition, compared with female HBV‐related HCC patients, the mutation percentage of COL6A6 was significantly increased in male HCC patients with HBV infection (Figure 5B). This evidence concerns the gene CPLANE1 and hepatocellular carcinoma.