Among these identified highly mutated genes, TP53, TERT, PEEX2, CTNNB1, and AXIN1 in HCC, especially in HBV‐related HCC, have been investigated, and the published studies showed that these mutated genes played very important roles in the development of HCC.10, 11, 18, 21 In addition, consistent with the published studies, we found that COL11A1, RB1, MUC16, and PCLO, which have been detected by next‐generation sequencing technology,13, 21, 22, 23 including whole genome sequencing and whole‐exome sequencing by different groups, are also found to be associated with HCC in our research. This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.