G-361 cells were treated with or without H2O2, or transfected with miR-517a inhibitor sequence, pcDNA-CDKN1C sequence, miR-517a mimic + pcDNA-CDKN1C sequence, SP600125 (JNK inhibitor), or relative negative control (NC) individually or together to identify their roles in OS of melanoma cells. The gene discussed is CDKN1C; the disease is melanoma.