We then treated mice with an NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), and utilized NF-κB knockout (p50-/-) mice to test whether the essential role of NF-κB in CNH-induced PAH depended on upregulation of miR-335-3p. The gene discussed is NFKB1; the disease is pulmonary arterial hypertension.