NFKB1 and pulmonary arterial hypertension: To further investigate the role of NF-κB in activating miR-335-3p transcription in the CNH-induced PAH mouse model in vivo, we performed qRT-PCR analysis of pri-miR-335-3p and miR-335-3p expression in CNH mouse lungs treated with the NF-κB inhibitor PDTC.