On the basis of the ROC curve, we obtained an optimum TBILA cut-off value of 0.923 (sensitivity, 64.7%; specificity, 80.7%) and AGAP2-AS1 cut-off value of 1.12 (sensitivity, 66.7%; specificity, 73.3%) for NSCLC diagnosis, respectively. The gene discussed is AGAP2; the disease is non-small cell lung carcinoma.