Primary AML cells from four patients (AML#1, AML#2, AML#3 and AML#4) with KMT2A-PTD/DNMT3A-WT and two patients (AML#5 and AML#6) with KMT2A-PTD/DNMT3A-MT were cultured in six-well plate. The gene discussed is DNMT3A; the disease is acute myeloid leukemia.