Tier 2 therapeutic relevant variants where found in seven different well-known cancer genes [22] (no. patients given in parenthesis): TP53 (8), EZH2 (8) KRAS (4), NRAS (3), TET2 (2), SRSF2 (1), NOTCH1 (1), and BRAF (1). The gene discussed is BRAF; the disease is cancer.