Testing both mRNA and protein levels, we observed that glucose transporter isoform 1 (GLUT1), pyruvate kinase isozymes M2 (PKM2) and monocarboxylate transporter 4 (MCT4) of OLEO-treated A375 melanoma cells are reduced by a 50% compared to control (i.e., untreated melanoma cells) (Figure 3). The gene discussed is PKM; the disease is melanoma.