IL1B and Hyperglycemia: Two sets of experiments were designed as followed: in vivo study was performed using a focal stroke model in T2DM mice, treated with or without rFGF21 as we previously described [10], and an in vitro study was conducted using cultured human brain microvascular endothelial cells (HBMECs), insulted by a well-established hyperglycemia plus interleukin (IL)-1β exposure model to mimic in vivo situation of diabetic stroke as we previously described [15].