In this work, we demonstrated for the first time in retinal whole mounts of a transgenic triple mouse 3xTg-AD that retinal microglial cells (Iba-1+ cells) show signs of activation in the different retinal sectors (superior, inferior, nasal, and temporal) of all retinal layers (OPL, IPL, and GCL/NFL) where these cells are found. This evidence concerns the gene AIF1 and Alzheimer disease.