Treatment of MDA-MB-231 breast cancer cells with rosemary extract (50 μg/mL) for 24 or 48 h resulted in a significant decrease in Akt phosphorylation/activation (25.62% ± 2.56% of control, p < 0.0001) and (11.05% ± 0.62% of control, p < 0.0001), respectively, (Figure 3a,b). This evidence concerns the gene AKT1 and breast cancer.