For 2D culture, the level of expression of COL1A1, COL5A1, FBLN1, IBSP, and TNMD was significantly higher in the DAF‐G and g‐DAF‐G groups than Col I group on Day 21, which indicated the potential induction of directing specific differentiation of hBMSCs towards AF cells on DAF‐G and g‐DAF‐G hydrogels (Figure 4b). Here, IBSP is linked to atrial fibrillation.