To determine the extent of full-length NKRF expression across cell types, extracts from cells representative of lung cancer (A549), breast cancer (MCF7), colorectal cancer (HT29), osteosarcoma (U-2 OS), cervical cancer (HeLa), neuroblastoma (SH-SY5Y), T-lymphocytes (Jurkat), macrophages (RAW264.7) and embryonic kidney (HEK293) were subjected to western analysis. The gene discussed is NKRF; the disease is osteosarcoma.