To asses this effect, we analyzed the expressions of p62, AKT, PI3K, mTOR, Beclin, Sestrin, and LC3 and observed the significant downregulation in p62, LC3, Beclin, Sestrin, mTOR, and PI3K in all cancer cells and upregulation of AKT. Further, for studying the effect of strophanthidin in MAPK signaling, p38MAPK, MEK1, MAPK24, SAPK/JNK, and p44 genes were selected. The gene discussed is MAPK9; the disease is cancer.