MT-ND1 and neoplasm: However, in the tumor antecedent, CZN5 tumor 1, the mt-ND1 (3274 T>TA) mutation frequency rose to 100% demonstrating that all mtDNA copies tested by NGS, from this tumor, possessed the mtND1(3274 T>TA) SNP variant in the same position in the mitochondrial coding region.