With the prophylactic protocol, we observed that pre-treatment of DCs with melanoma lysate did not increase the efficiency compared to Rb9 alone, suggesting that the most important priming occurred in vivo after challenge with B16F10 cells, possibly resulting in extensive cell lysis due to NK activity, perforins, and IFN-γ dependent and independent mechanisms (48). The gene discussed is PRF1; the disease is melanoma.