It is considered that gingival inflammation caused by poor toothbrushing is linked with increased systemic inflammation/endothelial dysfunction and low adiponectin levels, which in turn may lead to an increased risk of hyperglycemia.33 For individuals who had toothbrushing frequency of once a day without brushing teeth at night, increased frequency of toothbrushing may have the hidden potential to reduce a risk of hyperglycemia. This evidence concerns the gene ADIPOQ and endothelial dysfunction.