As shown in Fig. 1a–c, double-immunofluorescence staining for Iba1 (red) and GPER (green) in the hippocampal CA1 region revealed that ischemia/reperfusion (IR) 14 days induced a robust enhancement of Iba1 immunofluorescence intensity as well as higher co-localization (yellow) of Iba1 with GPER than that in non-ischemic (sham) animals. The gene discussed is AIF1; the disease is ischemia.