Therefore, IκBα/MPO, p65/MPO, p-p65/α-Tubulin, and p-IκBα/α-Tubulin were adopted to comprehensively analyze the relative phosphorylation, thereby indicating that QFAE regulates NF-κB signaling pathways by inhibiting the expression levels of p-IκBα and p-p65 caused by LPS in ALI mice. This evidence concerns the gene MPO and acute respiratory distress syndrome.