Future research could seek to confirm early FFV infection in qPCR-positive, ELISA-negative pumas by looking for anti-FFV IgM to confirm the qPCR as a true positive as well as quiescent FFV infection in qPCR-negative, ELISA-positive pumas by quantifying FFV RNA to confirm the ELISA was a true positive. The gene discussed is CD40LG; the disease is infection.