Confirming what was described in the previous experiments, analysis of T cell populations revealed increased expansion and persistence of adoptively transferred Thy1.1+ CD8 T cells in spleen and tumor of mice treated with ACT + NKTR-214 compared to ACT + IL-2, both at days 7 and 14 days (Fig. 4c, d, Supplementary Fig. 6b, c). This evidence concerns the gene CD8A and neoplasm.