Interestingly in our studies, the CD8/Tregs ratio was substantially increased in the tumor of mice treated with ACT + NKTR-214, while the ratio was much lower in the spleen, probably due to the homing and preferential expansion of specific pmel-1 effector T cells into the tumor as demonstrated here using several imaging and enumeration methodologies22. The gene discussed is CD8A; the disease is neoplasm.