Here, we demonstrated that the designed HTNV MAP could stimulate IFN-γ secretion from CD8+ T cells and induce strong proliferation of CD8+ T cells derived from HLA-A*02+ patients with HFRS ex vivo, suggesting HTNV MAP could effectively stimulate HTNV-specific CD8+ T cell of patients in vitro. Here, IFNG is linked to hemorrhagic fever with renal syndrome.