GRK7 and invasive breast carcinoma: For this experiment, we performed the 2-step PCR and LDR reactions as described above, using the primers for the detection of methylation at the CpG site located in the promoter region of the gene GRK7 (m_GRK7 or cg18768784; Chr3:_141516271–141,516,272), although highly methylated in the BrCa cohort, has low BRCA-specificity.