Next, to further verify the biological roles of PSMA3-AS1in esophageal cancer cells, we established stable PSMA3-AS1 knockdown via lentiviral infection in EC9706 and EC109 cell lines (which have high PSMA3-AS1 expression) and validated the down-regulation of PSMA3-AS1 by RT-qPCR (Figure 3A). The gene discussed is PSMA3; the disease is esophageal cancer.