PSMA3 and esophageal cancer: To further explore the biological roles of PSMA3-AS1 in esophageal cancer cells, we established stable PSMA3-AS1-overexpressing cell lines via lentiviral infection in KYSE150 and KYSE450 cell lines (which have low PSMA3-AS1 expression) and validated the up-regulation of PSMA3-AS1 by RT-qPCR (Figure 2A and 2B).