To investigate the roles of FANCA and FANCG in the FA pathway, and to better understand the FA- and cancer-associated mutations, we determined several cryo-electron microscopy (EM) structures of Xenopus laevis FANCA: the FANCA CTD alone at 3.35 Å and 3.46 Å resolution and the two distinct FANCA–FANCG complexes at 4.59 and 4.84 Å resolution, respectively (Figure 1; Supplementary Figures S1-2 and Table S1). Here, FANCG is linked to cancer.