Primary tumor cell line KPC1199 derived from KPC pancreatic ductal adenocarcinoma mouse (LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx-1-Cre, on C57BL/6 background) possess Kras and p53 double mutation, which reflect the most prevalent genotype of PDAC in clinic52. The gene discussed is KRAS; the disease is neoplasm.