CD4 and neoplasm: Higher TMB was correlated with higher T cell clonality (r = 0.19; p = 0.015, Spearman rank correlation), a lower CD4:CD8 ratio (r = −0.38; p = 0.0002, Spearman rank correlation), and higher GzmB (r = 0.32; p = 0.0019 by IHC, r = 0.26; p = 0.02 by gene expression profiling, Spearman rank correlation) (n = 215; Fig. 3a and n = 146; Supplementary Fig. 5B–G), supportive of the critical role of somatic mutations in enhancing tumor immunogenicity and triggering T cell responses through the generation of neoantigens.