By combining NPS with some specific biomarkers for osteosarcoma, such as miR-138-5p, circ_0000502, lncRNA TP73-AS1, and circ-NT5C2, numerous novel, specific, and multidimensional prognostic indexes can be formulated, but their prognostic performances need to be verified in future studies [10, 12, 66, 67]. The gene discussed is TP73; the disease is osteosarcoma.