Expect one SNV mutation of TP53 (c.G701A), we did not find other nonsynonymous mutations in high frequently mutated genes in HCC, including TP53, RB1, CDKN2A, VCX, TERT, AXIN1, SELPLG, ALB, FGF19, CNND1, RPS6KA3, CTNNB1, CDKN2A, and CDKN1B.[15] In contrast, copy number amplification of several key driver genes (BRCA1, ERBB2, FGFR1, NTRK1, TERT, and TP53) and several key functional genes (AKT2, ARID1A, CCND1, CDK4, FGF3, FGF4, FGFR3, IGF2, NOTCH1, PDGFRA, SRC, VEGFA, etc.)were observed. This evidence concerns the gene CDKN2A and hepatocellular carcinoma.