ZBTB7A and metabolic dysfunction-associated steatotic liver disease: Furthermore, since Kupffer and HSC cells are involved in the regulation of NAFLD, to evaluate whether ZBTB7A mainly functions in hepatocytes, the expression of ZBTB7A in primary hepatocytes, Kupffer, and HSC cells was subjected with the qRT-PCR assay, and the results showed that ZBTB7A was also expressed in the Kupffer and HSC cells, but highly expressed in the primary hepatocytes (Figure 6(d)).