The GBP1 gene KO DU145 and PC3 prostate cancer cells showed significantly less aggressive in vitro, i.e., less proliferation, infiltration, wound healing and colony formation capabilities with a prominent “anergy” status in both mitochondrial oxidative phosphorylation and glycolysis, and with a significantly higher sensitivity to chemotherapeutic reagents. The gene discussed is GBP1; the disease is Familial prostate cancer.