Paying attention to the increasing effect of MtS on HCC incidence and possibility of MtS factors contribution in this cancer, also, considering the Wnt signaling pathway involvement in HCC and liver lipid metabolism, and regulatory function of ANGPTL8 in the lipid metabolism and metabolic disorders, this study was conducted to investigate the possible effect of ANGPTL8 protein on the proliferation of the HCC cell line HepG2 and on the expression levels of two important Wnt signaling regulators β-catenin and WIF-1 in these cells. Here, WIF1 is linked to hepatocellular carcinoma.