CD8A and neoplasm: Unlike intratumoral donor OT-I CD8+ T cells, splenic-derived OT-I CD8+ T cells maintained cytokine production (51.18 ± 15.38% cytokine producing donors in spleens vs. 26.54 ± 14.38 cytokine producing donor cells in tumors) at day 22 post-tumor injection; and significantly more polyfunctional donor cells were also found in spleens (21.64 ± 9.149% vs. 12.96 ± 10.38%, p = 0.043) (Figure 4H).