Considering polyfunctional CD4+ T cells, the dominant responder population at 2 weeks post-infection for all groups combined and for historically BCG-vaccinated individuals alone (group C) was IFN-γ, TNF-α, and IL-2 triple-cytokine producing cells; while the second most abundant population was single IFN-γ producers (Figures 4A,B). Here, TNF is linked to infection.