An examination of the effect of lipofection with these six expression plasmids on [3H]-thymidine incorporation in U87 human glioma cells showed the maximal suppression of cell growth was obtained with a plasmid DNA that expressed an shRNA that targeted nucleotides 2,529–2,557 of the human EGFR mRNA, and this expression plasmid was designated clone 967 (Zhang et al., 2004b). Here, EGFR is linked to glioma.