Our results suggest that the oEnvB-RVΔG-DsRed-infected layer 5 neurons express oTVB-H in a Cre-dependent manner, probably because oTVB-H is markedly sensitive to oEnvB-RVΔG infection; even when the BFP reporter signal cannot be detected due to the low expression level of oTVB-H-BFP, oEnvB-RVΔG can still infect these cells. Here, RNF112 is linked to infection.