VIM and cancer: For example, four of the six ubiquitination sites identified in vimentin (VIME gene), a protein associated with epithelial-to-mesenchymal transition (EMT) that is upregulated across cancer types, exhibited an increase in ubiquitin occupancy with MG132 indicating these sites are responsible for signaling ubiquitin-mediated degradation of vimentin by the 26S proteasome (Additional file 1: Table S1) [13].