Notably, the tumors originating from OM-injected organoids are Otx2, NPR3 positive and GFAP, Olig2, Barhl1 negative (Fig. 5d; Supplementary Fig. 7A–D) similarly to the tumors induced by OM injection in CD1 mice (Fig. 2d, f, h; Supplementary Fig. 3D, F) and to human Group 3 MB27,28. The gene discussed is BARHL1; the disease is ocular melanoma.