PRNP and variant Creutzfeldt-Jakob disease: In parallel, 10−2 and 10−3 dilution vCJD-contaminated steel wires were implanted in tgHu (transgenic mice overexpressing the physiological level of human PrP by 6 times) mice (tg650 line) or otherwise used as the substrate for vCJD amplification by Surf-PMCA.