Moreover, in another BA model using the plant toxin biliatresone, toxin treatment caused extrahepatic cholangiocyte damage and fibrosis through decreased SOX17 expression in three-dimensional spheroid culture of mouse bile duct cells and neonatal extrahepatic duct cells (Waisbourd-Zinman et al., 2016). The gene discussed is SOX17; the disease is breast angiosarcoma.