DBA/SOX9 double whole-mount immunostaining revealed that DBA-positive materials (i.e. cellular debris) were frequently observed within the luminal space of both gallbladder and cystic duct in Sox17+/− neonates, but not in the healthy wild-type littermates (asterisks in Fig. 1B,C), indicating inflammation and scarring of fetal cholecystitis in Sox17+/− neonates (Higashiyama et al., 2017). This evidence concerns the gene SOX17 and Cholecystitis.