INS and metabolic syndrome: We evaluated Akt mobilization towards the plasma membrane by assessing its colocalization with caveolin-3, a muscle-specific integral membrane protein [39], after insulin stimulation for 2 min in control (Fig 3A) and MetS cardiomyocytes (Fig 3B) and found a significantly diminished colocalization coefficient in MetS (0.34 ± 0.04) vs control (0.57 ± 0.03) cardiomyocytes (P < 0.001, Fig 3C), without significant changes in the basal colocalization without insulin.