INS and metabolic syndrome: Here, we aimed to evaluate insulin-induced glucose uptake and Akt activation in cardiomyocytes of MetS rats, and also quantify changes in the abundance of proteins that form a complex with Akt by using a co-immunoprecipitation (CoIP) and label-free mass spectrometry approach, in order to provide proteomic support to the Akt activation status and to explore the molecular mechanisms related to Akt regulation that could be involved in the heart IR condition and the diabetic cardiomyopathy development.