The mPTP opening is a necessary process for this type of death pathways.48 The opening of mPTP not only provides a channel for Cyt C leakage but also leads to the collapse of ΔΨm and attracts PINK/Parkin aggregation, which finally induces mitophagy.49 Our current results indicated that there was a close relationship between mPTP opening and mitophagy under CTB treatment in HCC. The gene discussed is PRKN; the disease is hepatocellular carcinoma.