Activated AKT plays a strong anti‐apoptotic role in tumour cells with high glycolysis rate, and the binding of HK2‐VDAC1 is regulated by upstream Akt.32 To assess whether CTB can affect the activation of the Akt signalling pathway, Western blot and immunofluorescence were used to determine the expression of p‐Akt and Akt. The gene discussed is VDAC1; the disease is neoplasm.