Firstly, the levels of PI3K, AKT, p-PI3K, p-AKT, and p-PRAS40-Thr246 in gastric cancer cells and normal gastric mucosal epithelial cells were detected, and it was found that those levels in the gastric cancer cells were higher than those in normal gastric mucosal epithelial cells. The gene discussed is AKT1; the disease is gastric cancer.