This indicates that establishment of resident T cells from T cells originating from circulation may not be strictly dependent on neither α4 or CD103, but does involve CD69 in agreement with previous studies on CD4 Trm cells in various tissues46 including the genital tract.47 Interestingly, our data showed that inhibiting lymphocyte egress from the lymph nodes, including the infection-draining lymph nodes, resulting in a strong decline in T cell numbers in blood, did not reduce the expansion of T cells in the GT or compromize the protection. This evidence concerns the gene ITGAE and infection.