NR5A2 and alkaline phosphatase measurement: Moreover, the flow cytometric results showed that the proportion of EdU-positive cells was significantly reduced in Nr5a2 silenced cells treated with CAE (7.97 ± 0.36%) compared with cells treated with CAE only (51.48 ± 1.25%), and even the cells were almost at a standstill (Figures 3I,J), which indicated that the proliferation of acinar cells in AP model was markedly inhibited after silencing Nr5a2.