We found that high VGLL3 levels significantly associated with ECM receptor interaction, focal adhesion, pathways in cancer, MAPK signaling pathway, JAK STAT signaling pathway, ABC transporters, and the WNT signaling pathway, and that low expression levels associated with glyoxylate and dicarboxylate metabolism, DNA replication, glutathione metabolism, homologous recombination, and glycosylphosphatidylinositol gpi banchor biosynthesis. This evidence concerns the gene VGLL3 and cancer.