TP53 and neoplasm: We also applied unsupervised Louvain clustering of PTEN−/−;NF1−/− iHGG secondary tumors (Fig. 7a) and, in parallel, TP53−/−;PDGFRAΔ8–9 iHGG secondary tumors (Fig. 7b) to further compare inter- and intra-tumor variability of our iHGG tumor models and found 7 and 8 distinct clusters, respectively.